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KMID : 0364219890320030258
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Korean Journal of Zoology
1989 Volume.32 No. 3 p.258 ~ p.263
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Studies on the Improvement of Developmental Capadty of the Nucleocytoplasmic Hybrid by
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Chung Hae-Moon
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Abstract
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Nuclear Transplantation between Rana pipiens and Rana dybowskii When diploid blastula nuclei of Rana pipiens are traraplanted into enucleated eggs of Rana dybowskii the resulting nucleocytoplasmic hybrids are lethal-those development were arrested around the stage of the dorsal lip formation For the improvement of developmental capacity, serial nuclear transplantation was carried out. Even though serial transplantation of 15 generations showed normal development in each generation until gastrula stage, there was no sign of fundamental improvement in development afterward. This results implied that up to gastrulation normal DNA replication and cell division can take place in foreign cytoplasm. Since chromosomal aberrations both in shape and number were usually observed, the nuclei must have been modifted while resided in the foreign cytoplasm. Those nuclei didn't participate in normal development and led the embryos to early death. Tissue graft experiment indicated that the abnormal behavior of this lethal nucleocytoplasmic hybrid is an inherent property which is not corrected by the contact with its own tissue.
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KEYWORD
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Nucleocytpplasmic hybrid , Developmental capacity , Seral nuclear trans-plantation , Amphibian
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